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目的:建立人工蛹虫草药材的高效液相色谱指纹图谱。方法:收集11个不同产地的蛹虫草样品,用HPLC法测定色谱图,采用国家药典委员会颁布的“中药色谱指纹图谱相似度评价系统2004A版”软件生成指纹图谱,并对其进行相似度评价。结果:所建立的指纹图谱检测方法操作简单,重现性、稳定性和精密度均良好,得到具有14个共有峰的指纹图谱,各批样品图谱与对照指纹图谱的相似度均在0.9以上。结论:建立了准确、快速、简便的指纹图谱检测方法,所得到的指纹图谱适用于蛹虫草质量控制。
Objective: To establish a HPLC fingerprint of artificial Cordyceps militaris. Methods: Eleven samples of Cordyceps militaris were collected from different areas. The chromatograms were determined by HPLC. The fingerprint of the Chinese Medicines Chromatographic Fingerprinting Similarity Evaluation System 2004A was generated by National Pharmacopoeia Commission and the similarity was calculated Evaluation. Results: The fingerprint detection method was simple, reproducible, stable and accurate. The fingerprint with 14 common peaks was obtained. The similarity between each sample and the control fingerprinting was above 0.9. Conclusion: An accurate, rapid and simple fingerprint detection method was established. The obtained fingerprint was suitable for the quality control of Cordyceps militaris.