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目的比较细胞核阵列荧光原位杂交技术(FISH)和免疫组织化学在检测间变性大细胞淋巴瘤(ALCL)中间变性淋巴瘤激酶基因转位及其融合蛋白中的作用。方法联合应用细胞核阵列技术和双色FISH及免疫组织化学检测17例石蜡包埋ALCL病例中间变性淋巴瘤激酶基因转位及其融合蛋白。结果成功提取细胞核制成阵列去掉了胞浆对FISH的不良影响并提供了高通量的操作平台;总共17例标本中8例间变性淋巴瘤激酶免疫组织化学阳性的标本中,胞核胞浆均阳性4例,仅胞浆阳性4例;细胞核阵列FISH阳性结果6例,除了符合4例免疫组化胞浆胞核阳性的结果之外,还有2例免疫组化仅为胞浆阳性的标本FISH结果为阳性,剩余2例免疫组化仅为胞浆阳性的标本FISH结果为阴性。结论细胞核阵列FISH消除了细胞浆对FISH的不良影响,并提供高通量操作平台,使FISH成为临床检测的常用方法有了进一步的可能;FISH较免疫组化是更为特异的一个诊断方法。
Objective To compare the role of nuclear in situ hybridization (FISH) and immunohistochemistry in the detection of anaplastic lymphoma kinase gene translocation and its fusion protein in anaplastic large cell lymphoma (ALCL). Methods The nuclear translocation and fusion protein of 17 patients with paraffin-embedded ALCL were analyzed by nuclear array technology, two-color FISH and immunohistochemistry. Results Successful extraction of the nucleus into an array removed the unfavorable effects of cytoplasm on FISH and provided a high-throughput platform for operation. In a total of 17 specimens of anaplastic lymphoma kinase immunohistochemically positive specimens, nucleus cytoplasm 4 were positive, and only 4 were cytoplasm positive. FISH-positive results of nuclear array in 6 cases, in addition to the results of 4 cases of positive cytoplasmic nuclear immunohistochemistry, there are 2 cases of immunohistochemistry is only cytoplasm positive The FISH result of the specimen was positive, and the remaining 2 specimens with cytoplasmic positive staining were negative. Conclusion FISH can eliminate the unfavorable effects of cytoplasm on FISH and provide a platform for high-throughput operation, making FISH a common method for clinical detection. FISH is more specific than immunohistochemistry.