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目的:构建并评价一种CD44受体和叶酸受体双靶向的pH敏感纳米载体.方法:以pH敏感性的缩醛-薄荷缩酮(acetal-menthone 1,2-glycerol ketal,Ace-MGK)作为疏水端,寡聚透明质酸(oligomeric hyaluronic acid,oHA)和叶酸(folic acid,FA)分别作为CD44受体和叶酸受体靶标,合成具有智能化响应的多功能纳米靶向FoAM载体材料.通过傅里叶变换红外光谱法(IR)和核磁共振技术(1 H-NMR)对该载体材料进行结构鉴定.以姜黄素(curcumin,Cur)作为模型药物,通过透析法制得载姜黄素纳米胶束,并对其平均粒径、Zeta电位及形态进行表征.结果:成功合成FoAM载体材料,制备的FoAM载药胶束能够自组装为球形形态,平均粒径为158.2 nm,Zeta电位为-24.51 mV.结论:FoAM作为一种高效的纳米给药载体在抗肿瘤给药领域中具有潜在的应用前景.“,”Objective:To develop and evaluate dual CD44 receptor and folate receptor targeting pH-sensitive nanocarrier.Methods:The smart response muhifunctional oligomeric hyaluronic acid conjugate,folic acidoligomeric hyaluronic acid-acetal-menthone 1,2-glycerol ketal (FA-oHA-Ace-MGK),was synthesized by chemical reaction.The pH-sensitive Ace-MGK as hydrophobic moieties,oHA as the target of CD44 receptor and FA as the target of folate receptor.The structure of this dual CD44 receptor and folate receptor targeting smart response multifunctional nanocarrier was confirmed with Fourier-transform infrared and nuclear magnetic resonance (1H-NMR).The micelles of this nanocarrier were loaded with curcumin (Cur),a model drug,by dialysis method,and were characterized by mean diameter,zeta potential and morphology visualized by transmission electron microscopy (TEM).Results:FoAM nanocarrier was synthesized successfully.Drug-loaded FoAM could self-assemble to form spherical nanoparticles with the mean diameter of 158.2 nm and zeta potential of-24.51 mV.Conclusion:FoAM,as an effective nano-size carrier,has a promising potential for anti-tumor drug delivery.