用细胞核仁组成区相关蛋白染色与图像分析技术，研究γ干扰素（ＩＦＮγ）对正常和不同因子刺激的ＮＩＨ／３Ｔ３成纤维细胞相关嗜银蛋白（ＡｇＮＯＲｓ）的影响。发现（１）不同浓度的ＩＦＮγ对正常成纤维细胞的生长显示出明显的抑制作用，其中以２５０×１０３ｕ／Ｌ浓度ＩＦＮγ的抑制作用较强（Ｐ＜００１）；（２）ＩＦＮγ对肝素、５羟色胺和ＩＬ１刺激的成纤维细胞增殖的影响表明，２５０×１０３ｕ／Ｌ浓度的ＩＦＮγ对肝素和５羟色胺刺激的成纤维细胞显示出明显的抑制作用，其ＡｇＮＯＲｓ三个参数的均值相当于对照值的５４％和５１％，而２５０和５００×１０３ｕ／Ｌ浓度的ＩＦＮγ能明显抑制ＩＬ１刺激的成纤维细胞生长，其抑制率分别为对照值的６１％和５５％（Ｐ＜００１）；（３）比较ＩＦＮγ对正常和刺激生长的成纤维细胞的作用，发现ＩＦＮγ能不同程度地抑制成纤维细胞的生长，其中对５羟色胺刺激的成纤维细胞抑制作用最强，其次为正常和ＩＬ１刺激的成纤维细胞，而对肝素刺激的成纤维细胞增生的抑制作用较弱，其抑制率分别为对照值的６３％，６６％，７３％和８５％。以上结果为ＩＦＮγ治疗各种因素，特别是辐射引起的器官纤维化研究提供了? The effects of interferon-gamma (IFN-γ) on NIH / 3T3 fibroblast-associated argyrophilic proteins (AgNORs) stimulated by normal and different factors were studied by staining the nucleolar organizer region-related proteins and imaging analysis. The results showed that: (1) IFNγ at different concentrations showed obvious inhibitory effect on the growth of normal fibroblasts. The inhibitory effect of IFNγ at a concentration of 250 × 103u / L was stronger (P <001) ) IFN γ on heparin, 5 serotonin and IL 1 stimulation of fibroblast proliferation showed that the concentration of 250 × 103u / L IFN γ heparin and serotonin-stimulated fibroblasts showed significant inhibition , The average of AgNORs three parameters is equivalent to 54% and 51% of the control value, while the concentration of 250 and 500 × 103u / L of IFN γ can significantly inhibit the growth of IL 1 stimulated fibroblasts, the inhibition rates were (P <001). (3) The effect of IFNγ on normal and stimulated fibroblasts was compared, IFNγ was found to inhibit the growth of fibroblasts to some extent , Of which the inhibition of serotonin-stimulated fibroblasts strongest, followed by normal IL1 stimulated fibroblasts, whereas inhibition of heparin stimulated proliferation of fibroblasts weak inhibition rates were 63% of control values, 66%, 73% and 85%. The above results for the treatment of various factors IFN γ, especially radiation-induced organ fibrosis provided?