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目的 研究妥泰对沙土鼠脑缺血再灌注损伤的保护作用及作用机制。方法 采用沙土鼠双侧颈总动脉结扎制成的全脑缺血模型。56只沙土鼠随机分为假手术组、缺血组、治疗组和预防组。预防组的动物分别给予大、小剂量的妥泰(100 mg/kg、50mg/kg)灌胃观察3天而行手术,术后24小时处死动物;治疗组的动物术后立即分别给于大、小剂量的妥泰(100 mg/kg、50mg/kg)灌胃观察7天处死动物。测定各组沙土鼠血、脑组织中的MDA、SOD的含量。光镜和电镜下观察脑组织CA_1区的病理及超微结构改变。结果 与缺血组相比,妥泰治疗组和妥泰预防组脑组织中的MDA含量明显降低(P<0.01),而SOD含量明显增高(P<0.01)。光镜和电镜下观察发现,妥泰治疗组和妥泰预防组的脑CA_1区缺血改变较缺血组减轻,且大剂量组缺血改变明显减轻。结论 妥泰对沙土鼠脑缺血再灌注损伤具有良好的保护作用,且保护作用与妥泰的剂量大小有关。其脑保护作用机制之一为妥泰能有效抑制氧自由基的产生及毒性。
Objective To investigate the protective effect and mechanism of topiramate on cerebral ischemia-reperfusion injury in gerbils. Methods The model of global cerebral ischemia was made by ligating the common carotid arteries of gerbils. 56 gerbils were randomly divided into sham operation group, ischemia group, treatment group and prevention group. Animals in the prevention group were given large and small doses of topiramate (100 mg / kg, 50 mg / kg) orally to observe the operation for 3 days, and animals were sacrificed 24 hours after the operation. Animals in the treatment group were given large , Small dose of topiramate (100 mg / kg, 50mg / kg) was orally administered to observe the animals were sacrificed 7 days. The contents of MDA and SOD in blood and brain of each group were determined. The pathological and ultrastructural changes of CA 1 region were observed under light microscope and electron microscope. Results Compared with ischemic group, the content of MDA in brain tissue of Topiramate and Topirax group were significantly decreased (P <0.01), while the content of SOD was significantly increased (P <0.01). Light and electron microscopy showed that the hippocampal CA125 group and Topotecan group CA1 ischemic ischemic lesion than the ischemic group, and high-dose group was significantly reduced ischemic changes. Conclusion Topiramate has a good protective effect on gerbil brain ischemia-reperfusion injury, and its protective effect is related to the dose of Topiramate. One of the mechanisms of brain protection is that topiramate can effectively inhibit the production of oxygen free radicals and toxicity.