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目的用组织浴槽血管环研究蛇床子素对大鼠离体预收缩肺动脉环舒张作用的具体机制。方法游离健康SD大鼠的肺动脉血管,剪成长约3mm的血管环,以苯肾上腺素(1μM)预收缩后,探讨去除血管内皮、eNOS抑制剂L-NAME、各类钾通道抑制剂4-AP、glibenclamide、TEA、Ba Cl2对不同浓度蛇床子素对PE预收缩血管环的张力的影响。结果去除血管内皮及L-NAME能降低蛇床子素舒血管效应。4-AP(1mM)能降低蛇床子素舒血管效应,而glibenclamide(10μM)、TEA(10 mM)、BaCl2(100μM)对蛇床子素舒张预收缩大鼠离体肺动脉环的作用无明显影响。结论蛇床子素舒张大鼠离体肺动脉环的作用与内皮释放NO与Kv通道有关。
Objective To study the specific mechanism of ostium-ostium on the relaxation of pre-contracted pulmonary artery rings in rats by tissue bath vascular rings. Methods Pulmonary arterial vessels of healthy SD rats were isolated and cut into vascular rings of about 3 mm in length. After pre-shrinking with phenylephrine (1 μM), the effects of removing vascular endothelium, L-NAME, an inhibitor of 4-AP , Glibenclamide, TEA, Ba Cl2 on the tension of PE precontracted vascular rings at different concentrations of osthole. Results Removal of vascular endothelium and L-NAME decreased osthole vasorelaxant effects. However, glibenclamide (10μM), TEA (10mM) and BaCl2 (100μM) had no significant effect on the isolated pulmonary artery rings of ostholemic precontracted rats. Conclusion The effect of osthole-relaxing rat pulmonary rings on the release of NO and Kv channels in the endothelium.