论文部分内容阅读
为进一步了解和研究绵羊线粒体转录因子A(TFAM)基因的结构和功能,采用生物信息学方法构建了不同物种的TFAM基因系统发育树,并对绵羊TFAM基因编码蛋白质理化性质、二级结构、亚细胞定位、蛋白磷酸化、三级结构及功能结构域等进行分析。结果表明:绵羊和山羊亲缘关系最为接近,其次是家牛,与原鸡、斑马鱼亲缘关系较远。绵羊TFAM基因共编码246个氨基酸,以赖氨酸含量最高;二级结构以α-螺旋和无规则卷曲占为主;综合分析,此蛋白为碱性、不稳定性亲水蛋白,不存在信号肽和跨膜结构域;其主要在细胞核、线粒体、细胞骨架内发挥其生物学作用。该蛋白的丝氨酸磷酸化位点比较多,而酪氨酸和苏氨酸的磷酸化位点相对较少;且含有2个HMG结构域,在两个HMG结构域之间存在一个由12个氨基酸残基组成的短连接区域。
In order to further understand and study the structure and function of the sheep mitochondrial transcription factor A (TFAM) gene, bioinformatics methods were used to construct the phylogenetic tree of TFAM genes of different species. The physical and chemical properties, secondary structure, Cell localization, protein phosphorylation, tertiary structure and functional domains. The results showed that sheep and goat were the most closely related, followed by domestic cattle, which were distantly related to chicken and zebrafish. The sheep TFAM gene encodes a total of 246 amino acids, the highest lysine content; secondary structure of α-helix and random curly accounted for the main; comprehensive analysis of this protein is alkaline, unstable hydrophilic protein, there is no signal Peptides and transmembrane domains; they exert their biological functions primarily in the nucleus, mitochondria, cytoskeleton. This protein has many serine phosphorylation sites and relatively few tyrosine and threonine phosphorylation sites; and contains two HMG domains, and one protein consisting of 12 amino acids Residues composed of short connecting area.