论文部分内容阅读
应用AFLP技术对中间球海胆、光棘球海胆及杂交F1代(中间球海胆♀×光棘球海胆♂)群体的遗传多样性进行了分析。结果表明,4对引物共扩增得到272个位点,其中269个多态位点,总的多态位点比例为98.89%。3个群体的香农多样性指数分别为:0.2331±0.1273、0.2005±0.1385和0.2625±0.1067。群体内遗传相似度分别为:0.6876±0.0523、0.6501±0.0548和0.6552±0.0553。分子方差分析(AMOVA)结果表明,变异来源有25.39%来自群体间,有74.61%来自群体内,群体内的遗传多样性比较丰富。尽管杂交海胆在表型上可以明显分成两种类型,但是通过AFLP统计的遗传距离进行的个体聚类却随机聚在一起,不能分成两个群体。
The AFLP technique was used to analyze the genetic diversity of populations of sea urchin, Echinochloa crus-galli and F1 hybrids (Eichhornia crassipes × Echinochloa crusgalli ♂) population. The results showed that a total of 272 loci were amplified by 4 pairs of primers, of which 269 polymorphic loci had 98.89% of the total polymorphic loci. The Shannon diversity index of three populations were 0.2331 ± 0.1273, 0.2005 ± 0.1385 and 0.2625 ± 0.1067, respectively. The genetic similarity among groups were 0.6876 ± 0.0523, 0.6501 ± 0.0548 and 0.6552 ± 0.0553, respectively. Analysis of molecular variance (AMOVA) showed that 25.39% of the variation originated from the population, 74.61% came from the population, and the genetic diversity within the population was abundant. Although hybrid sea urchins can be clearly divided into two types phenotypically, individual clusters based on the AFLP statistical genetic distance are randomly clustered and can not be divided into two groups.