论文部分内容阅读
目的:研究芹菜素(API)与重组人可溶性肿瘤坏死因子相关凋亡诱导配体(TRAIL)合用对人宫颈癌HeLa细胞凋亡的影响。方法:体外培养人宫颈癌HeLa细胞。碘化丙啶(PI)染色流式细胞术(FCM)定量分析sub-G1百分率。DNA琼脂糖凝胶电泳观察细胞DNA梯形条带。结果:API(20μmol/L)和TRAIL(20ng/mL)以及两者合用作用48h的人宫颈癌HeLa细胞sub-G1百分率分别是3.56%±0.20%、6.69%±0.40%和59.8%±4.20%;DNA琼脂糖凝胶电泳显示:20μmol/LAPI与20ng/mL的TRAIL联合处理,展示出典型DNA梯形条带图谱。结论:芹菜素具有增强TRAIL诱导HeLa细胞凋亡作用。
Objective: To investigate the effect of API and recombinant human apoptosis-inducing ligand (TRAIL) on the apoptosis of human cervical cancer HeLa cells. Methods: Human cervical cancer HeLa cells were cultured in vitro. Propidium iodide (PI) staining flow cytometry (FCM) Quantitative analysis of sub-G1 percentage. The DNA ladder was observed by DNA agarose gel electrophoresis. Results: The percentage of sub-G1 of HeLa cells treated with API (20μmol / L) and TRAIL (20ng / mL) for 48h was 3.56% ± 0.20%, 6.69% ± 0.40% and 59.8% ± 4.20% ; DNA agarose gel electrophoresis showed that: 20μmol / LAPI and 20ng / mL of TRAIL combined treatment, showing a typical DNA ladder pattern. Conclusion: Apigenin can enhance the apoptosis of HeLa cells induced by TRAIL.