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目的:探讨姜黄素促人胃癌BGC-823细胞凋亡的生物学作用及其调控机制。方法:常规体外培养对数生长期胃癌BGC-823细胞,细胞分为对照组,低、中、高姜黄素处理组四组,姜黄素浓度分别为0 mg/L,5 mg/L,10 mg/L,20mg/L。姜黄素处理24 h后,采用甲基噻唑(MTT)比色法及流式细胞仪测定细胞增殖水平及细胞凋亡率;采用免疫组化法测定细胞内Bax、Bcl-2蛋白表达;采用PCR检测Caspase-3的mRNA表达水平。结果:MTT检测显示姜黄素能抑制人胃癌BGC-823细胞増殖,呈现浓度依赖性;流式细胞仪显示姜黄素能有效诱导细胞的凋亡,呈现浓度依赖性,其中20 mg/L姜黄素处理24 h后细胞凋亡率为48.3%;免疫组化试验表明姜黄素处理使人胃癌BGC-823细胞中Bax表达水平上调,同时Bcl-2蛋白表达水平下调;且细胞中Caspase-3的mRNA表达水平受姜黄素诱导而增高。结论:姜黄素对人胃癌BGC-823细胞的增殖具有明显抑制作用,呈浓度依赖性促进细胞凋亡,这种生物学效应可能与激活Bax蛋白表达、抑制Bcl-2蛋白表达而活化Caspase-3的信号通路有关。该研究为深入探讨姜黄素诱导人胃癌BGC-823细胞凋亡的机制提供了重要依据。
Objective: To investigate the biological role of curcumin in the apoptosis of human gastric cancer cell line BGC-823 and its regulatory mechanism. Methods: The BGC-823 cells were cultured in normal logarithmic growth phase. The cells were divided into four groups: control group, low, middle and high curcumin groups. Curcumin concentrations were 0 mg / L, 5 mg / L and 10 mg / L, 20 mg / L. After treated with curcumin for 24 h, MTT assay and flow cytometry were used to detect the cell proliferation and apoptosis rate. The expression of Bax and Bcl-2 protein was detected by immunohistochemistry. The mRNA expression level of Caspase-3 was detected. Results: MTT assay showed that curcumin could inhibit the proliferation of human gastric cancer cell line BGC-823 in a concentration-dependent manner. Flow cytometry showed that curcumin could induce cell apoptosis in a concentration-dependent manner. Curcumin at 20 mg / L After 24 h, the apoptosis rate was 48.3%. The results of immunohistochemistry showed that curcumin treatment up-regulated the expression of Bax and down-regulated the expression of Bcl-2 protein in BGC-823 cells. The mRNA expression of Caspase-3 Curcumin induced by the level of increased. CONCLUSION: Curcumin can significantly inhibit the proliferation of human gastric cancer cell line BGC-823 and promote the apoptosis in a concentration-dependent manner. This biological effect may be related to activating Bax protein expression, inhibiting Bcl-2 protein expression and activating Caspase-3 Of the signal path. This study provides an important basis for further exploring the mechanism of curcumin in inducing human gastric cancer cell line BGC-823 apoptosis.