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本研究分别用核酸杂交、免疫组织化学和电子显微镜术等方法从细胞、亚细胞和分子水平上比较分析了35例子宫颈增殖性病变中人乳头瘤病毒DNA相关序列,人乳头瘤病毒衣壳抗原的表达以及病变超微结构变化特征与不同型别人乳头瘤病毒感染之间的关系。结果表明:(1)宫颈湿疣多与人乳头瘤病毒11型(HPV—11)相关,宫颈癌主要与人乳头瘤病毒16型(HPV—16)相关;(2)子宫颈增殖性病变中人乳头瘤病毒衣壳抗原的表达与病变的恶性程度呈负相关,病变恶性程度愈高,人乳头瘤病毒衣壳抗原的表达愈低;(3)宫颈湿疣中HPV—11以诱导细胞的重度凹空病变为标志,HPV—16以引起细胞核的非典型性改变为特征;(4)人乳头瘤病毒衣壳抗原的表达主要见于HPV—11感染的具有凹空病变的宫颈湿疣中,而在HPV—16感染引起细胞核非典型性改变为特征的宫颈湿疣中,人乳头瘤病毒衣壳抗原的表达较低。以上结果表明:HPV—16致癌潜能较强。
In this study, nucleic acid hybridization, immunohistochemistry and electron microscopy and other methods were compared at the cellular, subcellular and molecular levels of 35 cases of cervical proliferative lesions of human papillomavirus DNA-related sequences, human papillomavirus capsid antigen And the relationship between ultrastructural changes and different types of human papillomavirus (HPV) infection. The results showed that: (1) Cervical condyloma was mostly associated with human papillomavirus type 11 (HPV-11), and cervical cancer was mainly associated with human papillomavirus type 16 (HPV-16); (2) Papillomavirus capsid antigen expression was negatively correlated with the degree of malignancy, the higher the degree of malignancy, the lower expression of human papillomavirus capsid antigen; (3) HPV-11 in cervical condyloma acuminatum to induce severe concave Empty lesions as a sign, HPV-16 to cause atypical changes in the nucleus is characterized by; (4) human papillomavirus capsid antigen expression mainly seen in HPV-11 infection with concave lesions of cervical condyloma, and HPV -16 infection caused by atypical changes in the nucleus characterized by cervical condyloma, human papillomavirus capsid antigen expression is low. The above results show that: HPV-16 strong carcinogenic potential.