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目的：观察人参对实验性缺血心肌细胞β受体及ｃ Ａ Ｍ Ｐ含量的影响。方法：对４５ 只健康成年 Ｓ Ｄ 纯种大鼠随机分为假手术对照组、单纯冠状动脉结扎组及冠状动脉结扎加人参治疗组，每组 １５ 只，应用受体放射分析法测定心肌细胞膜 β受体数量和解离常数（ Ｋｄ），放射免疫分析法测定各组心肌细胞内ｃ Ａ Ｍ Ｐ含量，在各组间进行比较。结果：结扎 Ｌ Ａ Ｄ２ 周后心肌细胞β受体的最大结合量（ Ｂｍ ａｘ，０．２７９ ｎｍ ｏｌ）较假手术组（０．０９３ ｎｍ ｏｌ）明显升高（ Ｐ ＜ ０．０５）；心肌细胞 β受体的 Ｋｄ（１２．４３１ ｎｍ ｏｌ）较假手术组（１．３１９ ｎｍ ｏｌ）明显升高（ Ｐ ＜ ０．０５）；心肌细胞内ｃ Ａ Ｍ Ｐ水平〔（１ ２９３．９６±５１９．３６） ｐｍ ｏｌ／ｇ〕亦明显高于假手术组〔（７７４．４４±２１０．５５） ｐｍ ｏｌ／ｇ〕（ Ｐ ＜ ０．０１）；经人参治疗 ２周后缺血心肌内 ｃ Ａ Ｍ Ｐ 水平〔（８０５．０２±３６２．４８） ｐｍ ｏｌ／ｇ〕明显低于单纯冠状动脉结扎组（ Ｐ ＜０．０１），与假手术组相似。结论：人参有降低缺血心肌内ｃ Ａ Ｍ Ｐ水平的作用。
Objective: To observe the effect of ginseng on β-receptor and c A M P content in experimental ischemic myocardial cells. METHODS: Forty-five healthy adult SD rats were randomly divided into sham operation control group, coronary artery ligation group, coronary artery ligation and ginseng treatment group, 15 rats in each group. The myocardial cell membrane β was measured by receptor radioactivity assay. The number of receptors and the dissociation constant (Kd) were determined by radioimmunoassay. The content of c A M P in myocardial cells in each group was determined and compared between groups. RESULTS: The maximum binding of β-adrenoceptor (Bm ax, 0.279 nmol) was significantly higher in the ventricular cardiomyocytes after lA D2 occlusion than in the sham-operated group (0.093 nmol) (P < 0.05); The Kd (12.431 nmol) of β-adrenoceptor was significantly higher than that of sham-operated group (1.319 nmol) (P < 0.05); c A M P level in cardiomyocytes [(1 293.96±) 519.36) pm ol/g was also significantly higher than that of sham group ((774.44±210.55) pm ol/g) (P < 0.01); myocardial ischemia was observed 2 weeks after ginseng treatment. The A M P level ((805.02±362.48) pm ol/g) was significantly lower than that of the pure coronary artery ligation group (P < 0.01), similar to the sham operation group. Conclusion: Ginseng can reduce the level of cAMP in the ischemic myocardium.