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将 2 -氨乙基硫醇 (AET)固载到玻碳电极 (GCE)表面 ,进而化学吸附纳米金 (NG) ,并在纳米金上固载ss-DNA得到 ss-DNA/NG/AET/GCE,以 Co(bpy) 3+ 3为电化学指示剂可以识别研究 ss-DNA的杂化反应 .结果表明 ,纳米金能使固载其上的 ss-DNA发生部分变性而结合 Co(bpy) 3+ 3,但用 p H7.0的磷酸缓冲液浸泡可基本上避免这种变性 ,并明显提高杂化反应的识别能力 .结合在 ds-DNA/NG/AET/GCE上的 Co(bpy) 3+ 3的峰电流与扫速的线性关系可保持到 80 m V/s.与电沉积法固载纳米金相比较 ,本电极更稳定和可靠 .
The AET was immobilized on the surface of glassy carbon electrode (GCE), and then the gold nanoparticles (NG) were chemisorbed. The ss-DNA was immobilized on gold nanoparticles to obtain ss-DNA / NG / AET / GCE, the hybridization reaction of ss-DNA can be identified by using Co (bpy) 3+ 3 as electrochemical indicator.The results show that the gold nanoparticles can partially denature ss- 3+ 3, but this denaturation was substantially avoided by soaking in phosphate buffer pH 7.4 and significantly improved the recognition ability of the hybridization reaction.Co (bpy), which binds to ds-DNA / NG / AET / GCE, The linear relationship between the peak current of 3+ 3 and the scan rate can be maintained to 80 mV / s. The electrode is more stable and reliable than electrodeposited gold nanoparticles immobilized.