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[目的]木薯单爪螨是08年新入侵我国的重要害螨,在非洲、南美等木薯种植国家曾导致严重损失甚至绝收,目前我国相关研究几乎空白。温度是影响木薯单爪螨生长发育与繁殖的重要生态因子之一。为了解木薯单爪螨入侵中国后的适生性分布,解析其入侵、扩散及生态适应性机制,本文研究了极端高温对木薯单爪螨几种保护酶活性的影响,以为其监测预警与有效防控奠定基础。[方法]分别将木薯单爪螨幼螨、前若螨、后若螨和成螨置于42℃高温人工气候箱中处理不同时间(0.5,1,2,4,8,16and24h)后取存活个体,采用分光光度计终点测定法测定了42℃极端高温处理各龄期螨不同时间后其体内几种保护酶(多酚氧化酶PPO、过氧化物酶POD、抗坏血酸氧化酶AsA-POD、过氧化氢酶CAT、超氧化物歧化酶SOD和酯酶EST)活性的变化。[结果]结果表明,经42℃极端高温胁迫,木薯单爪螨幼螨和雌成螨的PPO、POD、AsA-POD、CAT活性显著增加、前若螨和后若螨CAT活性显著下降及其PPO、POD、AsA-POD活性的稳定性可能与木薯单爪螨耐热性相关。[结论]木薯单爪螨各龄螨经42℃极端高温胁迫后体内保护酶PPO、POD、AsA-POD以及CAT活性的变化可能与木薯单爪螨对高温的耐受性存在显著相关性。木薯单爪螨为新入侵我国热区的害螨,长期高温条件胁迫可能会导致其耐热性个体逐渐增多,种群耐热性增强,进而导致种群迅速扩张
[Objective] The single-legged mite of Cassava is the new important pest that invaded China in 2008. In Africa, South America and other countries where cassava was planted, it caused serious loss or even failure. At present, the related research in our country is almost blank. Temperature is one of the important ecological factors that affect the growth and reproduction of Monascus mite. In order to understand the physiological distribution of Monascus mites after its invasion to China and analyze its mechanism of invasion, spread and ecological adaptation, this paper studied the effects of extreme high temperatures on the activities of several protective enzymes of Monascus mites. Control laid the foundation. [Method] The larvae, anterior pomes, procreate nymphs and adult mites of Carassius monojaponica were placed in the high temperature artificial climate chamber at 42 ℃ for different times (0.5, 1, 2, 4, 8, 16 and 24 hours) Individuals using the spectrophotometer endpoint determination of 42 ℃ treatment of extreme high temperature treatment of various age mites after several protective enzymes (polyphenol oxidase PPO, peroxidase POD, ascorbate oxidase AsA-POD, Catalase CAT, superoxide dismutase SOD and esterase EST) activity changes. [Result] The results showed that the activity of PPO, POD, AsA-POD and CAT increased significantly under the extreme high temperature stress of 42 ℃, the activity of CAT and the activity of PPO, POD, AsA-POD and CAT decreased significantly The stability of PPO, POD and AsA-POD activities may be related to the heat tolerance of Monascus mites. [Conclusion] The changes of the activities of PPO, POD, AsA-POD and CAT in the endoparasites of Monochoria mites at 42 ℃ under high temperature stress may have significant correlation with the high temperature tolerance of Monascus mites. Cassia uniportal is the new mite that invaded the hot area of our country. Under long-term high temperature stress, the heat-resistant individuals may gradually increase, and the heat tolerance of the population will increase, leading to the rapid population expansion