论文部分内容阅读
本文目的在于观察体外培养神经元在兴奋性改变状况下其Agrin表达的变化,藉以了解Agrin与神经元发育的关系。采用高钾和低钾培养基培养皮质神经元,用免疫组织化学方法观察这些神经元Agrin表达的变化程度。结果表明,与正常组相比,高钾培养的皮质神经元在形态上无明显改变,神经元胞体的大小、突起的长短都与对照组接近;低钾培养神经元也仍能保持正常的形态,未发现明显的形态改变。免疫细胞化学结果提示,在高钾和低钾培养的皮质神经元中Agrin表达都明显增强,但高钾组的表达程度较低钾组为高。结论:神经元培养液中钾离子浓度所致的神经元活动是影响Agrin表达的重要因素。
The purpose of this paper is to observe the change of Agrin expression of neurons cultured in vitro under excitatory changes in order to understand the relationship between Agrin and neuronal development. Cortical neurons were cultured in high potassium and low potassium medium, and the changes of Agrin expression in these neurons were observed by immunohistochemistry. The results showed that compared with the normal group, the cortical neurons cultured in high potassium had no morphological changes, the size of the neuronal soma and the length of the protuberances were close to those in the control group. The neurons in hypokalemia maintained normal morphology , No obvious morphological changes were found. Immunocytochemistry showed that Agrin expression was significantly increased in both high potassium and low potassium cultured cortical neurons, but higher in the high potassium group than in the low potassium group. Conclusion: Neuronal activity induced by potassium concentration in neuronal cultures is an important factor affecting Agrin expression.