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目的 探讨体外培养内皮细胞植于蛋白涂层支架 ,使支架完全内皮化的可行性。方法 4只镍钛记忆合金支架明胶蛋白涂层 ,置猪髂动脉内皮细胞悬液中吸附 2h ,6孔培养板中培养 1周 ,倒置显微镜观察细胞生长情况。体外模拟血管内支架输送系统输送支架后 ,观察细胞在支架的贴附状态。结果 1周后支架完全内皮化 ,支架经输送系统后 ,80 %细胞贴附支架上 ,培养 72h后 ,支架上附着细胞恢复输送前状态。结论 蛋白涂层支架完全可内皮化 ,对于其预防急性血栓形成和再狭窄的价值有待于进一步研究。
Objective To investigate the feasibility of culturing endothelial cells in protein-coated scaffolds in vitro and making the scaffold completely endothelialized. Methods Four NiTi memory alloy scaffolds were coated with gelatin protein. The cells were fixed in porcine iliac artery endothelial cell suspension for 2h and cultured in 6-well plate for 1 week. The growth of the cells was observed under inverted microscope. In vitro simulation of vascular stent delivery system delivery stent, the observation of cells attached to the stent state. Results After 1 week, the scaffolds were completely endothelialized. After the scaffolds were delivered by the delivery system, 80% of the cells were attached to the scaffolds. After 72 hours of culture, the adherent cells on the scaffolds restored the state before delivery. Conclusion The protein-coated scaffolds are completely endothelialized, and their value in preventing acute thrombosis and restenosis remains to be further studied.