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PAS方法创立近70年来,用于组织切片证明多糖类微生物的存在具有很高的实用价值,在组织病理学诊断及多糖类微生物的研究中起了重要作用。但背景中的非特异性染色难以克服;1946年Gomori发表了甲基胺-硝酸银法,其改良方法用于多糖微生物的染色,但背景很重。1988年日本Tome等学者提出用氢氧化氨-硝酸银法显示多糖类微生物,经我们应用并行部分改进,认为是比较理想的方法。 1 方法和步骤 (1)组织经10％的福尔马林固定,石蜡包埋,切片。(2)脱蜡,经各级酒精入水(氨银液升
PAS method was founded nearly 70 years ago, the tissue section proved the presence of polysaccharides has a high practical value in the diagnosis of histopathology and polysaccharides research has played an important role. Non-specific staining in the background was difficult to overcome; in 1946 Gomori published methylamine-silver nitrate method, an improved method for the staining of polysaccharide microorganisms, but with a heavy background. In 1988, Tome et al. Proposed that ammonium polysulfate-silver nitrate should be used to display polysaccharides, which is considered to be an ideal method after some parallel improvements were applied. 1 Methods and Procedures (1) Tissue was fixed in 10% formalin, embedded in paraffin and sectioned. (2) dewaxing, after all levels of alcohol into the water (ammonia silver liquid liter