生发中心含有大量增殖B细胞(CD77~+),少量T细胞(CD57~+/CD4~+)和滤泡树突状细胞(FDc,HJZ+)。其中CD57+TH细胞几乎仅存在于生发中心。为了研究细胞因子对生发中心微环境中的B细胞发育所起的作用,搞清生发中心中细胞因子的来源,对10种和B细胞增殖、分化密切相关的细胞因子(IL一la,IL一ip,iL一2,IL一3,IL一4,IL一5,IL一6,IL一10,TNF一a和IFN一Y)的mRNA在上述生发中心细胞中的表达进行了检 The germinal center contains a large number of proliferating B cells (CD77 ~ +), a small number of T cells (CD57 ~ + / CD4 ~ +) and follicular dendritic cells (FDc, HJZ +). Among them, CD57 + TH cells exist almost exclusively in the germinal centers. In order to study the role of cytokines in the development of B cells in the germinal center microenvironment and to clarify the origin of cytokines in the germinal center, the cytokines (IL-1a, IL-1 IL-3, IL-4, IL-5, IL-6, IL-10, TNF-α and IFN-γ mRNA in the above germinal center cells were examined