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目的 探讨前列腺特异抗原 (PSA)mRNA在前列腺癌 (PCa)患者外周血表达的意义。方法 运用巢式RT PCR检测 36例PCa患者 ,2 8例良性前列腺组织增生 (BPH)患者和 15例健康志愿者外周血。结果 BPH患者和志愿者外周血PSAmRNA均阴性 ,36例PCa外周血PSAmRNA阳性率 38 9% (14 / 36 )。PSAmRNA表达阳性率随肿瘤分期进展而增高 ,各期阳性率有显著性差异 (P <0 0 5 )。结论 PSAmRNA特异表达于PCa外周血 ,可用作PCa微转移的指标
Objective To investigate the significance of prostate specific antigen (PSA) mRNA expression in peripheral blood of patients with prostate cancer (PCa). Methods Nested RT-PCR was used to detect the peripheral blood of 36 PCa patients, 28 benign prostatic hyperplasia (BPH) patients and 15 healthy volunteers. Results The PSA mRNA in peripheral blood of BPH patients and volunteers was negative. The positive rate of PSA mRNA in 36 PCa peripheral blood samples was 38.9% (14/36). The positive rate of PSA mRNA expression increased with the progress of tumor staging, the positive rate of each stage was significantly different (P <0 05). Conclusion PSA mRNA is specifically expressed in PCa peripheral blood and can be used as an indicator of PCa micrometastasis