百日咳毒素(PTX)对百日咳具有重要的免疫原性,经化学灭活的PTX菌苗对儿童具有高度的保护作用。然而经化学减毒处理后的PTX,要求不降低其活性是很困难的,而且还有毒性逆转的可能性。为了寻找一种低毒性菌苗,作者对用基因诱变得到的百日咳杆菌TOX3201菌株产生的PTX类似物CRM3201,进行了一系列的免疫学和生物学的试验,证明这种PTX类似物可能作为一种侯选菌苗。作者从百日咳杆菌T1和3779菌株制备天然PTX,从TOX3201菌株制备CRM3201。并 Pertussis toxin (PTX) is important for pertussis immunization. Chemically inactivated PTX vaccines are highly protective of children. However, the chemically attenuated PTX requires that it is difficult to reduce its activity without further toxicity reversal. In order to find a low-toxic bacterin, the authors conducted a series of immunological and biological tests on the PTX analogue CRM3201 produced by genetically mutated Bordetella pertussis TOX3201 strain, demonstrating that this PTX analogue may serve as a Candidate bacterins. The authors prepared native PTX from B. pertussis T1 and 3779 strains and CRM3201 from TOX3201 strain. and