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目的:探讨胞二磷胆碱对1-甲基-4-苯基吡啶(MPP+)诱导的多巴胺能神经元数量减少及形态学改变的影响。方法:体外原代培养怀孕第14天小鼠胚胎中脑神经元,采用MPP+(10μmol·L-1)在体外建立多巴胺能神经元损伤细胞模型。实验分为对照组、单纯MPP+药物组及5个不同浓度胞二磷胆碱(0.01、0.10、1.00、10.00和100.00μmol·L-1)与MPP+共同给药组。应用酪氨酸脱氢酶免疫化学染色方法观察不同浓度胞二磷胆碱对MPP+诱导多巴胺能神经元损伤的形态及数量的变化。结果:体外培养第12天,单纯MPP+药物组中多巴胺能神经元的数量明显少于对照组(P<0.05);0.10μmol·L-1和100.00μmol·L-1胞二磷胆碱治疗组,多巴胺能神经元的数量分别高于单纯MPP+药物组(11.83%和21.26%)(P<0.05)。0.10和100.00μmol·L-1胞二磷胆碱加MPP+药物组中多巴胺能神经元的突起长度明显高于单纯MPP+药物组(P<0.05)。结论:胞二磷胆碱可有效地防止MPP+的多巴胺能神经元的损伤和死亡,可能为帕金森氏病的防治提供一个新途径。
AIM: To investigate the effects of citicoline on the number and morphological changes of dopaminergic neurons induced by 1-methyl-4-phenylpyridine (MPP +). Methods: Primary cultured mouse embryonic midbrain neurons were cultured in vitro on day 14 of pregnancy. MPP + (10 μmol·L -1) was used to establish a model of dopaminergic neuron injury in vitro. The experiment was divided into control group, MPP + drug group and 5 different concentrations of citicoline (0.01,0.10,1.00,10.00 and 100.00μmol·L-1) co-administered with MPP + group. The tyrosine dehydrogenase immunochemical staining method was used to observe the changes of morphology and quantity of MPP + -induced dopaminergic neurons with different concentrations of citicoline. Results: On the 12th day in vitro, the number of dopaminergic neurons in the MPP + drug group was significantly less than that in the control group (P <0.05). In the 0.10μmol·L-1 and 100.00μmol·L-1 citicoline treatment groups , The number of dopaminergic neurons were higher than the MPP + group (11.83% and 21.26%, respectively) (P <0.05). Protrusion length of dopaminergic neurons in 0.10 and 100.00 μmol·L-1 citicoline plus MPP + group was significantly higher than that in MPP + group (P <0.05). Conclusion: Citicoline can effectively prevent the injury and death of dopaminergic neurons in MPP +, which may provide a new way for the prevention and treatment of Parkinson ’s disease.