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目的以金纳米粒子修饰的氧化铟锡(ITO)玻璃为基底电极,将多壁碳纳米管-普鲁士蓝-壳聚糖(MWNTs-PB-CHIT)纳米复合物及蛋白A(PA)共修饰于其表面固载抗体制得高灵敏甲胎蛋白(AFP)电流型免疫传感器。方法先在ITO电极表面电沉积一层纳米金,随后将MWNTs-PB-CHIT纳米复合物滴涂于ITO玻璃电极的纳米金膜上,最后利用壳聚糖的吸附性及在酸性(pH=6.5)环境的负电性,静电吸附蛋白A并定向固定甲胎蛋白抗体(anti-AFP),制得电流型免疫传感器。结果在最佳实验条件下,该传感器响应的峰电流值与AFP浓度在1.0～250.0ng/ml的范围内保持良好的线性关系,检测下限为0.5ng/ml,反应达到稳定电流所用时间为6min,并且电流值相对标准差小于3%。结论成功构建一次性使用、价格低廉的新型传感器电极材料。
OBJECTIVE To modify the multi-walled carbon nanotubes-Prussian blue-chitosan (MWNTs-PB-CHIT) nanocomposites and protein A (PA) with gold nanoparticle modified indium tin oxide (ITO) Immobilized antibody on its surface to produce highly sensitive AFP (AFP) current type immunosensor. The method comprises the following steps: firstly, electrodepositing a layer of nano gold on the surface of the ITO electrode, then dripping the nano-composite of MWNTs-PB-CHIT on the nano gold film of the ITO glass electrode; finally, utilizing the adsorption property of the chitosan and the pH value of 6.5 ) Negative environment, electrostatic adsorption of protein A and directional immobilization of anti-AFP antibody (anti-AFP), the current-type immunosensor. Results Under the optimal experimental conditions, the peak current value of the sensor response and the AFP concentration in the range of 1.0 ~ 250.0ng / ml to maintain a good linear relationship, the detection limit of 0.5ng / ml, the reaction time to reach a steady current of 6min , And the relative standard deviation of current is less than 3%. Conclusion The successful construction of a one-time use, low price of the new sensor electrode material.