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目的 探讨胃癌及癌旁组织中HPV 16的感染及 p2 1基因突变是否存在协同致癌作用及其与胃癌患者预后的关系。方法 采用多聚酶链反应技术检测 46例胃癌及癌旁组织标本中HPV 16型感染 ,并采用免疫组织化学链霉菌抗生物素蛋白 过氧化酶连接法对胃癌 p2 1基因突变进行检测。结果 HPV16阳性率为 41.30 % ( 19/4 6) ,p2 1阳性表达为 5 2 .17% ( 2 4/4 6)。随访表明 46例有 2 1例出现复发或远处转移 ,其中HPV16阳性者复发率为 73.68% ( 14 /19) ,p2 1基因阳性表达者复发率为 66.60 % ( 16/2 4)。结论 HPV 16感染可能是胃癌发生的病因之一 ,而 p2 1基因失活具有协同致癌作用 ,亦可作为判断胃癌预后的指标之一。
Objective To investigate whether there is a synergistic carcinogenic effect of HPV 16 infection and p21 gene mutations in gastric cancer and paracancerous tissues and its relationship with the prognosis of gastric cancer patients. Methods Polymerase chain reaction (PCR) was used to detect HPV 16 infection in 46 specimens of gastric cancer and paracancerous tissues. P21 mutations in gastric cancer were detected by immunohistochemical streptavidin peroxidase linkage assay. Results The positive rate of HPV16 was 41.30 % (19/46), and the positive rate of p21 was 52.17% (2 4/46). Follow-up showed that 46 cases had recurrence or distant metastasis in 21 cases, of which the recurrence rate of HPV16-positive was 73.68% (14/19), and the recurrence rate of p21 gene positive expression was 66.60 % (16/2 4). Conclusion HPV 16 infection may be one of the causes of gastric cancer. The inactivation of p21 gene has a synergistic carcinogenic effect and can be used as an indicator of the prognosis of gastric cancer.