雌甾酚酮(1)是合成甾体口服避孕药的原料之一,目前最简便的合成方法是用节杆菌(Corynebacterium simplex转化19-羟甲基-~4-雄甾烯-3,17-双酮(2),2由去氢表雄酮乙酸酯(3)经五步化学反应制得。已知节杆菌能使甾体C_3-乙酸酯水解成羟基并氧化羟基为酮。本文报道以19-羟甲基-去氢表雄酮乙酸酯(4a)为原料,用节杆菌转化成1.由于从3合成4a只需三步化学反应,因此比从3合成2又可省去两步化学反应。4a Estrone (1) is one of the raw materials for the synthesis of steroidal oral contraceptives. At present, the easiest synthesis method is to use the Corynebacterium simplex to transform 19-hydroxymethyl- ~ 4-androstene-3,17- Diketone (2), 2 was prepared by a five-step chemical reaction of dehydroepiandrosterone acetate (3), which is known to hydrolyze the steroidal C 3 -acetate to a hydroxyl group and to oxidize the hydroxyl group to a ketone. Reported 19-hydroxymethyl-dehydroepiandrosterone acetate (4a) as raw material, with Arthrobacter transformed to 1. Since the synthesis of 4a from 3 requires only three steps of chemical reaction, so than 3 from the synthesis of 2 can be saved Go to two steps of chemical reaction .4a