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本文报道了酸樱桃微型繁殖的方法,筛选出较适宜的试管苗增殖和生根的培养基配方。增殖培养基为 MS+0.5PPm6—BA+0.1PPm 生物素、MS+0.75PPm6—BA+0.2PPmIA A+0.1PPm 生物素,蔗糖均为3%。生根培养基为 MS+0.2 PPmIAA+0.1PPm 生物素+3%蔗糖、1/2MS+0.5PPmIBA+2%蔗糖。芽苗增殖率40天达到4倍以上,无根苗25天生根率达到75%,通过对试管苗移栽生理的研究,和移栽技术的改进,使移栽成活率超过80%,至今已获得移栽成活苗1543株.
This paper reports the method of sour cherry mini-propagation, and screened more suitable culture medium for the proliferation and rooting of in vitro plantlets. The proliferation medium was MS + 0.5PPm6-BA + 0.1PPm biotin, MS + 0.75PPm6-BA + 0.2PPmIA A + 0.1PPm biotin, 3% sucrose. The rooting medium was MS + 0.2 PPmIAA + 0.1 PPm biotin + 3% sucrose, 1/2 MS + 0.5 PPmIBA + 2% sucrose. The proliferation rate of buds reached more than 4 times in 40 days, and the rooting rate of rootless seedlings reached 75% in 25 days. The survival rate of transplanting was over 80% through the physiological study on the transplanting of seedlings and the improvement of transplanting techniques 1543 plants transplanted live seedlings.