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目的:研究复发性流产患者和正常人工流产妇女绒毛组织中TLR4和NF-κB p65的表达差异及二者是否存在相关性。方法:选择2011年5~11月在解放军第202医院妇产科门诊、年龄25~35周岁、孕龄<12周、≥3次自然流产的RSA患者30例作为实验组,有过正常生育史、要求人工流产终止妊娠的健康早孕妇女30例作为对照组。应用免疫组织化学法检测两组绒毛中TLR4和NF-κB p65的表达,采用RT-PCR法检测两组绒毛组织中TLR4和NF-κB p65 mRNA的表达。结果:与对照组比较,实验组HE染色的病理观察绒毛组织结构不清晰,可见结构缺损、水肿、变性以及炎性细胞浸润,滋养细胞亦见变性、坏死及明显增生;TLR4和NF-κBp65的蛋白及mRNA的表达均明显升高,差异有统计学意义(P<0.01)。结论:TLR4、NF-κB p65在妊娠的绒毛组织中蛋白和mRNA表达水平显著升高,可能预示TLR4-NF-κB信号传导通路与RSA发生密切相关,其机制有待于进一步研究。
OBJECTIVE: To investigate the differences of TLR4 and NF-κB p65 expression in chorionic villi between recurrent spontaneous abortion and normal abortion women and whether they are related to each other. Methods: From May to November in 2011, 30 cases of RSA patients with obstetrics and gynecology clinic of the 202 Hospital of PLA, 25-35 years of age, gestational age <12 weeks, and ≥3 times of spontaneous abortion were selected as the experimental group and had normal fertility history , Requiring abortion termination of pregnancy healthy pregnant women 30 cases as a control group. The expressions of TLR4 and NF-κB p65 in both groups were detected by immunohistochemistry. The expressions of TLR4 and NF-κB p65 mRNA in both groups were detected by RT-PCR. Results: Compared with the control group, the histological observation of the HE staining in the experimental group showed that the villus tissue structure was unclear. The structure defects, edema, degeneration and infiltration of inflammatory cells were observed. The degeneration, necrosis and hyperplasia of trophoblastic cells were also observed. The expressions of TLR4 and NF- Protein and mRNA expression were significantly increased, the difference was statistically significant (P <0.01). CONCLUSION: The expressions of TLR4 and NF-κB p65 protein and mRNA in pregnancy villi are significantly increased, which may indicate that TLR4-NF-κB signaling pathway is closely related to RSA. The mechanism remains to be further studied.