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目的探讨不同人巨细胞病毒糖蛋白B基因分型与婴幼儿感染致病间的关系。方法临床采集200例婴幼儿感染HCMV血液标本,采用巢式PCR检测130例婴儿肝病综合征患者、33例血小板减少症患者和37例神经性耳聋患者标本中HCMV的gB基因,并通过限制性片段长度多态性分析(RFLP)对gB基因进一步分型。分析HCMV及其不同gB基因型与上述婴幼儿感染致病的关系。结果婴儿肝病综合征患者中gB基因型主要以gB1型为主,占56.9%;血小板减少症患者中gB基因型分布较均衡,各gB分型无特异性;而在神经性耳聋患者中,gB基因型主要为gB4型,占40.5%,其次为gB1型占24.3%。不同gB基因型HCMV感染患者在婴儿肝病综合征和神经性耳聋中的分布差异有统计学意义(P<0.05)。结论婴儿肝病综合征和神经性耳聋与HCMV的gB分型密切相关,而血小板减少症无直接关系。
Objective To investigate the relationship between genotyping of different human cytomegalovirus glycoprotein B and the pathogenesis of infant infection. Methods HCMV blood samples were collected from 200 infants and toddlers. Nested PCR was used to detect the gB gene of HCMV in 130 infants with hepatopathy syndrome, 33 patients with thrombocytopenia and 37 patients with neurological deafness. The length polymorphism analysis (RFLP) further genotyped the gB gene. To analyze the relationship between HCMV and different gB genotypes and the above-mentioned infantile infection pathogenesis. Results The gB genotype of infants with hepatopathy syndrome was predominantly gB1, accounting for 56.9%. The genotype of gB genotype was more balanced in all patients with thrombocytopenia and the genotypes of gB were nonspecific. In patients with neurological deafness, gB The genotype was mainly gB4, accounting for 40.5%, followed by gB1 accounting for 24.3%. The distribution of HCGV infection in different genotypes of gB in infants with hepatopathy syndrome and neurological deafness was significantly different (P <0.05). Conclusion Infantile hepatopathy syndrome and neurological deafness are closely related to gB genotyping of HCMV, while thrombocytopenia is not directly related.