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目的:探讨慢性应激对心肌梗死后大鼠模型行为学及海马BDNF的影响。方法:建立急性心肌梗死模型,结合慢性不可预见的轻度应激和孤养制作心梗并抑郁复合大鼠模型。观察动物的体重变化和行为学指标,用Western-blot方法检测海马BDNF蛋白表达。结果:经过21天慢性不可预见轻度应激,模型组大鼠体重、糖水消耗和糖水偏爱百分比、敞箱试验得分均明显降低,纯水消耗显著增加;海马BDNF蛋白表达减少(P<0.05)。结论:对急性心肌梗死大鼠采用21天慢性应激后,模型鼠体重下降、行为学明显异常,符合抑郁动物的行为学改变,且存在海马神经元可塑性降低的表现,这可能是心肌梗死并抑郁发病的机制之一。
Objective: To investigate the effects of chronic stress on behavior and hippocampal BDNF in rats after myocardial infarction. Methods: Acute myocardial infarction model was established. Combined with chronic unpredictable mild stress and solitary, myocardial infarction and depression model rats were made. The body weight changes and behavioral indexes of the animals were observed. The expression of BDNF protein in hippocampus was detected by Western-blot. Results: After 21 days of chronic unpredictable mild stress, the body weight, the percentage of sugar consumption, the percentage of sugar preference, the open-box test scores and the water consumption of model rats significantly increased. The expression of BDNF protein in the hippocampus decreased (P <0.05) . Conclusion: After 21 days of chronic stress in acute myocardial infarction rats, the body weight of the model rats is decreased and the behavior is obviously abnormal, which is in line with the behavioral changes of the depressed animals and shows the plasticity of the hippocampal neurons. This may be the result of myocardial infarction Depression, one of the mechanisms.