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目的:通过采用小鼠淋巴瘤细胞实验微孔法,检测二甲基亚砜(DMSO)的细胞毒性及遗传毒性,探索其在检测系统中的适宜浓度。方法:在非代谢活化(-S9)条件下,检测美国Sigma和Tedi A两家公司生产的2个批号DMSO,终浓度0.5%,1.0%,2.0%,4.0%处理L5178Y细胞3 h后产生的细胞毒性及诱发的TK基因突变频率(MF)。结果:2个批号DMSO的细胞毒性均以1%浓度组为最低(<20%),4%浓度组为最高(>37%);1%浓度组诱发的MF与空白对照组相近,而其他3组则明显增加(P<0.05或0.01)。结论:当以DMSO为溶媒、采用L5178Y细胞进行MLA微孔法实验时,采用1%作为检测系统中受试物的体积比浓度较为适宜。
OBJECTIVE: To investigate the cytotoxicity and genotoxicity of dimethyl sulfoxide (DMSO) in mouse lymphoma cells and to explore its suitable concentration in the detection system. Methods: Two batches of DMSO produced by Sigma and Tedi A were tested under the condition of non-metabolic activation (-S9). The final concentration of DMSO was 0.5%, 1.0%, 2.0% and 4.0% Cytotoxicity and induction of TK gene mutation frequency (MF). Results: The cytotoxicity of 2 batches of DMSO was the lowest (<20%) at the concentration of 1% and the highest at the concentration of 4% (> 37%). The MF induced by 1% concentration was similar to that of the blank control group, while the others 3 group was significantly increased (P <0.05 or 0.01). CONCLUSIONS: When using DMSO as solvent and L5178Y cells for MLA micropore assay, it is appropriate to use 1% as the volume concentration of the test substance in the detection system.