论文部分内容阅读
目的建立黑蒴茎芽愈伤组织诱导、分化及生根组织培养快繁体系。方法以黑蒴带节嫩茎芽为外植体,以MS(1/2MS)为基本培养基,考察不同质量浓度6-BA、NAA对愈伤组织诱导、分化及茎芽生根的影响。结果芽茎诱导愈伤的最佳培养基为MS+6-BA 1.0 mg/L+NAA 1.0 mg/L+Bn80g/L+Pt50g/L+25g/L蔗糖+6g/L琼脂,愈伤诱导率71.2%;愈伤分化不定芽最佳培养基为1/2MS+6-BA 0.5 mg/L+NAA 0.3 mg/L+Bn80g/L+Pt50g/L+25g/L蔗糖+6g/L琼脂,芽诱导率为84.2%;茎芽生根培养基为1/2MS+NAA 0.1~0.3 mg/L+Bn80g/L+Pt50g/L+25g/L蔗糖+6g/L琼脂,生根率超过87.2%。结论筛选出茎芽诱导愈伤、分化不定芽、生根的培养基,建立了黑蒴嫩茎芽组织培养快繁体系。
Objective To establish callus induction, differentiation and rapid propagation of rooting tissue culture in the stem of black bolt. Methods The stems and buds of A. nigra were used as explants. MS (1/2 MS) was used as the basic medium to study the effects of different concentrations of 6-BA and NAA on callus induction, differentiation and rooting of shoots. Results The best medium for callus induction was MS + 6-BA 1.0 mg / L + NAA 1.0 mg / L + Bn 80 g / L + Pt 50 g / L + 25 g / L sucrose + 6 g / L agar. 71.2%. The optimal medium for adventitious bud differentiation was 1 / 2MS + 6-BA 0.5 mg / L + NAA 0.3 mg / L + Bn80g / L + Pt50g / L + 25g / L sucrose + 6g / The induction rate was 84.2%. The rooting rate of shoots was 1 / 2MS + NAA 0.1 ~ 0.3 mg / L + Bn80g / L + Pt50g / L + 25g / L sucrose + 6g / L agar. Conclusion The buds of callus induction, differentiation of adventitious buds and rooting were screened out, and the tissue culture system was established.