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M852-1是经杂交和回交培育的普通小麦-柔软滨麦草易位系,苗期对我国小麦条锈菌流行小种均表现良好抗性。为明确其抗条锈性遗传规律,本研究选用条锈菌流行小种(类型)CYR29、CYR32、CYR33和Su11-7的单孢菌系对其与铭贤169杂交F1、F2、F3及BC1代群体进行遗传分析,同时应用420对SSR引物对接种CYR32的M852-1/铭贤169 F2代144个单株作图群体进行抗病基因定位。结果表明,M852-1对供试小种均表现免疫或近免疫,对CYR29的抗锈性由1对显性基因控制,对CYR32、CYR33和Su11-7的抗锈性均由1对隐性基因控制。筛选到3个与抗CYR32基因连锁的SSR标记Xbarc124、Xbarc200和Xgwm429,遗传距离分别为6.3、5.6和9.7 cM。根据SSR标记锚定性将该基因定位于小麦2BS染色体,暂命名为YrM852。基因来源、分子标记检测及染色体位点分析表明,YrM852很可能是1个不同于目前已知抗条锈病基因的新基因。
M852-1 is a common wheat-softwood wheat translocation line that is cross-fertilized and backcrossed. The seedling stage showed good resistance to the wheat stripe rust race in China. In order to clarify the genetic rule of resistance to stripe rust, F1, F2, F3 and BC1 of F1 hybrids with Mingxian 169 were selected from the monospora of CYR29, CYR32, CYR33 and Su11-7 At the same time, 420 pairs of SSR primers were used to map the resistance genes of 144 individuals of F2 population of M852-1 / Mingxian 169 inoculated with CYR32. The results showed that M852-1 was immunized or nearly immunized against the tested races. The rust resistance of CYR29 was controlled by one pair of dominant genes. The resistance to CYR32, CYR33 and Su11-7 was affected by one pair of recessive Gene control. Three SSR markers Xbarc124, Xbarc200 and Xgwm429 linked to anti-CYR32 gene were screened with genetic distances of 6.3, 5.6 and 9.7 cM, respectively. According to the anchorage of SSR markers, the gene was located in the wheat 2BS chromosome and tentatively named YrM852. Gene source, molecular marker detection and chromosomal location analysis indicated that YrM852 is probably a new gene different from the currently known stripe rust resistance genes.