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目的:准确分析一起细菌性食物中毒的原因,为食物中毒处理提供依据。方法:按照GB/T4789.10-94《食品卫生微生物学检验》和WS/T80-1996《葡萄球菌食物中毒诊断标准及处理原则》方法进行检验,分析中毒的原因。结果:3家幼儿园当天饮用的乳制品均检出金葡菌肠毒素,其它食品样中未检出;患儿呕吐物中有5份分离培养出金黄色葡萄球菌,分离菌株中有2份检出金葡菌肠毒素,其中3株经同源性分析认为同一来源;2份乳品厂工人手面破损涂抹样分离出金黄色葡萄球菌,分离菌株均检出金葡菌肠毒素,2份原料罐中的生鲜奶样品中均分离出金黄色葡萄球菌,分离菌株均检出金葡菌肠毒素,所有样品均未检测出其它致病菌;菌株经不同增菌液培养后肠毒素的产生有差异,胰酪大豆肉汤产毒率最高为66.7%(6/9),脑心浸液产毒率为44.4%(4/9),普通肉汤中为11.1%(1/9)。结论:病原学结果证实本起食物中毒由饮用金葡菌肠毒素污染的乳制品引起。
Objective: To accurately analyze the causes of bacterial food poisoning and provide basis for food poisoning treatment. Methods: According to GB / T4789.10-94 “Food Hygiene Microbiology Test” and WS / T80-1996 “Staphylococcus aureus food poisoning diagnostic criteria and principles of treatment” method for testing to analyze the causes of poisoning. Results: Staphylococcus aureus enterotoxin was detected in the drinking milk products of the three kindergartens, but not detected in other food samples. Staphylococcus aureus was isolated from five of the vomitus in children and two in the isolates Staphylococcus aureus enterotoxins, of which three homologous analysis that the same source; 2 dairy workers hand-side smear samples isolated Staphylococcus aureus, Staphylococcus aureus enterotoxin isolates were detected, two raw materials Staphylococcus aureus was isolated from the sample of fresh milk in the tank, Staphylococcus aureus enterotoxin was detected in all isolates, and no other pathogenic bacteria were detected in all the samples. The differences were as follows: the highest rate of toxin production was 66.7% (6/9) in soy broth, 44.4% (4/9) in brain heart infusion, and 11.1% (1/9) in common broth. CONCLUSIONS: The etiological results confirm that the food poisoning was caused by dairy products contaminated with Staphylococcus aureus enterotoxins.