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目的:观察天麻钩藤饮大孔树脂不同分离部位对偏头痛模型大鼠的影响。方法:按随机数字表法将70只SD大鼠随机分为阳性对照组(西比灵组)、正常对照组、模型对照组、天麻钩藤饮(TMGTY)组、天麻钩藤饮30%part组(以下简称30%part组)、天麻钩藤饮60%part组(以下简称60%part组)、天麻钩藤饮90%part组(以下简称90%part组)共7组。采用皮下注射硝酸甘油注射液复制偏头痛大鼠模型;采用时间分段计数法持续观察造模后大鼠耳红出现和消失时间、挠头等行为学的变化情况;采用酶免法测定各组大鼠血清5-羟色胺(5-HT)和肾上腺素(E)含量。结果:正常对照组未出现耳红症状,其余各组均出现耳红症状(P<0.01)。除正常对照组外,各组耳红出现时间整体比较,差异无统计学意义(P>0.05)。与模型对照组比较,TMGTY组、60%part组的耳红消失时间均缩短(P<0.05);30%part组、90%part组大鼠耳红消失时间虽有缩短趋势,但差异无统计学意义(P>0.05)。模型对照组在皮下注射10 mg/kg硝酸甘油注射液后30~150 min的挠头次数较正常对照组增加(P<0.01)。与模型对照组比较,60%part组在0~150 min内不同时间段的挠头次数均减少(P<0.05,P<0.01);TMGTY组仅在30~60 min时间段挠头次数减少(P<0.05);30%part组和90%part组仅在120~150 min时间段时间段挠头次数减少(P<0.05)。与正常对照组比较,模型组对照组的血清5-HT含量降低(P<0.01),血清E含量升高(P<0.05)。与模型对照组比较,TMGTY组、60%part组和90%part组的血清5-HT水平均升高(P<0.01),30%part组的血清5-HT水平有升高趋势,但差异无统计学意义(P>0.05);TMGTY组、30%part组、60%part组和90%part组的血清E水平均下降(P<0.05,P<0.01)。结论:天麻钩藤饮60%乙醇洗脱部位能有效改善模型大鼠耳红、挠头等症状,调节模型大鼠的血清5-HT、E含量。
Objective: To observe the effect of Tianma Gouteng decoction macroporous resin on different migraine models in migraine model rats. Methods: Seventy SD rats were randomly divided into positive control group (xibinger group), normal control group, model control group, TMGTY group and Tianma Gouteng Decoction 30% part Group (hereinafter referred to as 30% part group), Tianma Gouteng Decoction 60% part group (hereinafter referred to as 60% part group) and Tianma Gouteng 90% part group (hereinafter referred to as 90% part group) for a total of 7 groups. Subcutaneous injection of nitroglycerin injection of migraine rat model; continuous use of time-counting method to observe the occurrence and disappearance of the rat after the formation of ear redness, scratching head behavioral changes; enzyme immunoassay was used to determine the large group Rat serum serotonin (5-HT) and epinephrine (E) content. Results: The normal control group did not show the symptoms of otitis. The other groups showed the symptoms of otitis (P <0.01). Except the normal control group, there was no significant difference in the appearance time of each group of ear red (P> 0.05). Compared with the model control group, the disappearance time of ear red in TMGTY group and 60% part group was shortened (P <0.05). The disappearance time of ear red in 30% part group and 90% part group was shortened, but there was no statistical difference Significance (P> 0.05). The number of scratching of the model control group 30 ~ 150 min after subcutaneous injection of 10 mg / kg nitroglycerin injection was higher than that of the normal control group (P <0.01). Compared with the model control group, the number of scratching in 60% part group decreased in different time periods from 0 to 150 min (P <0.05, P <0.01), while the number of scratching in TMGTY group decreased only in 30-60 min (P < 0.05). The number of scratching of the head in 30% part group and 90% part group decreased only in 120 ~ 150 min time period (P <0.05). Compared with the normal control group, the serum 5-HT level in the model group decreased (P <0.01) and the serum E level increased (P <0.05). Compared with the model control group, serum 5-HT levels in TMGTY group, 60% part group and 90% part group were significantly increased (P <0.01), serum 5-HT level in 30% part group was increased, but the difference There was no significant difference between the TMGTY group, the 30% part group, the 60% part group and the 90% part group (P <0.05, P <0.01). CONCLUSION: 60% ethanol elution from Tianma Gouteng Decoction can effectively improve the symptoms of ear and scratching head, and regulate the content of 5-HT and E in serum of model rats.