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目的:探讨DC的抗肿瘤免疫功能是否与其基因位点有关。方法:选择19株基因型明确的BXD纯系小鼠的树突状细胞(DC),MTT法和ELISA分析对DC吞噬肿瘤细胞的作用;肿瘤细胞诱导:DC产生IL-12的能力;经肿瘤细胞刺激后的DC对T细胞增殖和:IFN-γ分泌的影响进行测定。用流式细胞仪检测:DC表面抗原标志CD80和CD54。有关数据用MapManagerQTX and WebQTL软件对DC抗肿瘤免疫的基因位点进行分析。结果:DC刺激T细胞增殖的作用很大程度取决于BXD各系的遗传差异。DC吞噬肿瘤细胞及诱导IL-12产生的能力都与DC刺激T细胞的增殖相一致(P<0.01)。基因位点定量分析(QTL)分析在第6和第13号染色体上有2个位点可能与DC的肿瘤抗原提呈加工功能和T细胞毒作用有关。结论:不同系的BXD小鼠,其DC的抗原提呈、加工能力不同。DC对肿瘤细胞生长的抑制与IL-12的诱导产生和DC表面共刺激分子的表达呈平行。其有关基因位点分别于第6和第13号染色体上。
Objective: To investigate whether the anti-tumor immune function of DC is related to its gene locus. Methods: Dendritic cells (DCs) of 19 BXD mice with genotypes were determined by MTT assay and ELISA. The effect of DC on phagocytosis of tumor cells was observed. Tumor cells induced the ability of DCs to produce IL-12. The effect of DC-stimulated DC on T cell proliferation and IFN-γ secretion was determined. Detection by flow cytometry: DC surface antigen markers CD80 and CD54. Relevant data The gene locus of DC anti-tumor immunity was analyzed with MapManagerQTX and WebQTL software. Results: The effect of DC stimulating T cell proliferation depends largely on the genetic differences among BXD lines. The ability of DCs to phagocytose tumor cells and induce the production of IL-12 are consistent with the proliferation of DC-stimulated T cells (P <0.01). Quantitative analysis of gene loci (QTL) analysis of two loci on the 6th and 13th chromosomes may be related to the tumor antigen presenting function and T-cell cytotoxicity. Conclusion: Different strains of BXD mice have different DC antigen presentation and processing ability. The inhibition of tumor cell growth by DC was parallel to the induction of IL-12 production and the expression of co-stimulatory molecules on the DC surface. The relevant gene locus on the 6th and 13th chromosome respectively.