本文对44例甲胎蛋白(AFP)检测阴性而临床疑为肝癌的患者作了细针肝穿刺细胞学检查,其中40例获得了细胞学阳性结果;肝癌阳性率91％;经细胞学检查发现AFP阴性者常存在于高分化型和低分化型两种肝癌病例中。本文还介绍了细针肝穿刺的操作方法和体会。但穿刺是否会影响预后问题值得注意。 In this paper, fine needle aspiration biopsy was performed on 44 patients with negative AFP detection and clinically suspected liver cancer, of which 40 cases had positive cytological findings; the positive rate of liver cancer was 91%; cytological examination revealed AFP-negative persons are often present in both well-differentiated and poorly-differentiated liver cancer cases. This article also introduced the operation method and experience of fine needle liver puncture. However, whether the puncture will affect the prognosis is worth noting.