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培养于附加2,4-D、BA和KT的愈伤组织诱导培养基上的火炬松成熟合子胚在培养3-9周后形成白色、半透明、有光泽的粘性愈伤组织。这类愈伤组织形成于成熟合子胚的子叶,但当用NAA或者IBA代替愈伤组织诱导培养基中的2,4-D时,它的诱导频率明显降低。这种粘性愈伤组织在分化培养基上形成体细胞胚。体细胞胚经过去50μm ABA和8.5%PEG600处理后成为耐干化胚。扫描电镜观察表明,萌发处理36小时后,耐干化胚恢复到干化处理之前的状态且大小和形态正常,而不耐干化胚不能恢复到干化处理之前的状态且表面撕裂。过氧化物酶活性的分析结果表明,耐干化胚有更高的过氧化物酶活性。耐干化胚的高过氧化物酶活性可能与催化H2O2的分解和保护体细胞胚免受氧化的伤害有关。
Pinus taeda mature zygotic embryos cultured on callus induction medium supplemented with 2,4-D, BA and KT formed white, translucent, shiny, adherent callus after 3-9 weeks of culture. This type of callus is formed on the cotyledons of mature zygotic embryos, but its induction frequency is significantly reduced when NAA or IBA is used instead of 2,4-D in the callus induction medium. This sticky callus forms somatic embryos on differentiation media. Somatic embryos became resistant to dry embryos after being treated with 50μm ABA and 8.5% PEG600. Scanning electron microscopy showed that after 36 hours of germination treatment, the embryogenic calli returned to their pre-desiccation state with normal size and morphology, while the desiccated embryos failed to recover to their pre-desiccation state and surface tearing. The analysis of peroxidase activity showed that the dry embryos had higher peroxidase activity. The high peroxidase activity of dry-resistant embryos may be related to the catalytic decomposition of H2O2 and the protection of somatic embryos against oxidative damage.