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我们于1985年秋用“龙衣”“银霞冠”“风凰振羽”“绿牡丹”等10个菊花品种的花蕾和“凤凰振羽”的根蘖芽进行了试管培养育苗试验。具体方法 (1)以花蕾作试材:取直径0.1—1.0厘米的花蕾,用0.1%升汞液消毒7—10分钟,在无菌条件下将花蕾切成四块接种到诱导发芽培养基上,在25℃左石和1,000勒克司光照条件下进行培养。(2)以根蘖芽作试材:于秋季菊花发出根蘖芽时,将芽剪下,用清水冲洗掉浮土,再用0.1%升汞液消毒7—10分钟,並在无菌条件下用高压灭菌过的普通水冲洗2—3次,剪成1—2厘米长的小段,接种于培养
In the autumn of 1985, we used the flower buds of 10 varieties of chrysanthemums such as “Longyin”, “Yinxia crown”, “Fenghuangyu feather” and “Green peony”, and the root tiller of “Fenghuangyuanyu” Bud test tube culture nursery test. Specific methods (1) to bud as a test material: Take the diameter of 0.1-1.0 cm flower buds, sterilized with 0.1% mercuric chloride solution for 7-10 minutes, the buds were cut under sterile conditions into four inoculated to the induction of germination medium , Cultured at 25 ° C on stone and 1,000 lux light. (2) The root tiller buds for the test material: in the autumn chrysanthemum issued when the root tiller buds, cut the buds, rinsed with water off the soil, and then sterilized with 0.1% mercury solution 7-10 minutes, and under sterile conditions Rinsed with autoclaved normal water 2-3 times, cut into 1-2 cm long pieces, inoculated in culture