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[目的]进一步揭示甘蓝型油菜和诸葛菜属间远缘杂种高世代(F8～F10)材料的遗传变异规律。[方法]以甘蓝型油菜和诸葛菜属间杂种高世代群体为材料,对甘蓝型油菜和诸葛菜远缘杂交后代表型偏白菜型油菜变异群体进行形态、细胞学方面的研究。[结果]形态学分析表明,杂种后代叶型、叶色、株型、早花性状偏向白菜型油菜,而二级分枝数、角果长度和千粒重与甘蓝型油菜Oro更为接近;细胞学研究表明,这些杂种高世代材料细胞中已不含诸葛菜染色体,多数植株都为少于38条染色体的亚倍体;与白菜型油菜杂种的减数分裂配对结果显示,这些亚倍体后代细胞中丢失的可能是来自C基因组的染色体,随着世代的增加,体细胞染色体数目都有向甘蓝型油菜2n=38升高和回归的趋势。[结论]该研究为揭示杂种后代亚倍体中丢失染色体的来源及形态学改变的原因奠定基础。
[Objective] The aim of this study was to further reveal the genetic variation of distant hybrids (F8 ~ F10) between Brassica napus and. [Method] With morphological and cytological studies on the mutant population of the representative Chinese cabbage rapeseed (Brassica napus L. and O. [Result] Morphological analysis showed that leaf type, leaf color, plant type and early flowering trait of hybrid progeny were more apt to Brassica campestris. However, the number of secondary branches, the length of pod fruit and the 1000 - grain weight were closer to Oro of Brassica napus. Studies have shown that these hybrids of high generations of material cells have no Dictyotrichia chromosomes, most of the plants are less than 38 chromosomes of the diploid; and Chinese cabbage rape hybrid meiotic pairing results show that these sub-dorsal progeny cells The chromosomes in C genome may be lost. With the increasing of generations, somatic chromosome number tends to increase and return to 2n = 38 in Brassica napus. [Conclusion] The study laid the foundation for revealing the source of chromosome loss and morphological changes in the diploid progeny.