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目的 研究重庆地区 1998～ 2 0 0 0年度秋冬季婴幼儿轮状病毒腹泻分子流行病学。方法 采用逆转录 聚合酶链反应 (RT PCR)扩增婴幼儿腹泻便样中的编码轮状病毒VP7蛋白的全基因片段 (10 6 2bp) ,再用巢式 聚合酶链反应 (net PCR)对扩增得到的VP7基因进行分型。同时利用核苷酸序列分析方法进行分型。结果 在 1998～ 1999年度 130例婴幼儿腹泻便样中VP7基因阳性者 5 0例 (38.46 % ) ,其中G1型占 88% (4 4/5 0 ) ,G3型占 8% (4 /5 0 ) ,混合型占 4% (2 /5 0 ) ,均为G1+G3型 ;而 1999～ 2 0 0 0年度轮状病毒流行季节采集的 112份标本中VP7基因扩增阳性者 38例(33.93% ) ,其中G3型占 78.95 % (30 /38) ,G1型占 13.16 % (5 /38) ,混合型占 7.89% (3/38) ,均为G1+G3型。核苷酸序列分型结果与PCR分型结果一致。结论 重庆地区 1998～ 1999年度轮状病毒流行季节中流行的轮状病毒以G1型为主 ,而 1999～ 2 0 0 0年度轮状病毒流行季节中G3型为主 ,在连续两年的监测中出现轮状病毒血清型的转变
Objective To study the molecular epidemiology of infant rotavirus diarrhea in autumn and winter in Chongqing from 1998 to 2000. Methods RT-PCR was used to amplify the complete genome of rotavirus VP7 protein (106bp) in infantile diarrhea samples. The nested PCR (nested PCR) The amplified VP7 gene was typed. At the same time using nucleotide sequence analysis method for typing. Results Fifty cases (38.46%) of VP7 positive cases were detected in 130 cases of infantile diarrhea in 1998 ~ 1999, of which 88% (4 4/5) in G1 type and 8% (4/500) in G3 type ), And mixed type accounted for 4% (2/50), all of which were G1 + G3 type. Of the 112 specimens collected during the rotavirus season from 1999 to 2000, 38 (33.93 %). The G3 type accounted for 78.95% (30/38), the G1 type accounted for 13.16% (5/38), and the mixed type accounted for 7.89% (3/38), both of which were G1 + G3 type. Nucleotide sequence typing results were consistent with PCR typing. Conclusion The majority of rotavirus circulating in the epidemic season of rotavirus from 1998 to 1999 in Chongqing was G1, while G3 was predominant in the epidemic season of rotavirus from 1999 to 2000. During the two consecutive years of monitoring A rotavirus serotype shift occurs