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目的:探讨二烯丙基二硫(DADS)对体外培养的人白血病细胞系K562细胞生长阻抑和凋亡作用及机制。方法:采用MTT分析法检测细胞活性、流式细胞术分析细胞周期及凋亡率、免疫组化检测p21WAF1基因表达。结果:1).DADS在10mg/L～80 mg/L范围内,对K562细胞的抑制作用呈剂量-时间依赖效应;2).不同浓度DADS作用于K562细胞24h后,细胞周期发生了变化:DADS可以将K562细胞阻滞于G2/M期;3).DADS浓度在10mg/L～80mg/L时作用K562细胞24h后,凋亡率逐渐升高,有显著的统计学意义(P<0.05或P<0.01);4).用浓度分别为0mg/L,20mg/L,40mg/L,80mg/L处理K562细胞24h后,p21WAF1蛋白表达上调,有统计学意义(P<0.05或P<0.01),溶媒组和阴性对照组无差别(P>0.05)。结论:DADS有抑制K562细胞增殖和促进K562细胞凋亡的作用。其作用的可能机制与上调细胞周期蛋白依赖性激酶抑制剂p21WAF1表达,从而诱使k562细胞阻滞于G2/M期有关。
Objective: To investigate the effect of diallyl disulfide (DADS) on the growth inhibition and apoptosis of human leukemia cell line K562 cultured in vitro and its mechanism. Methods: The cell viability was detected by MTT assay. The cell cycle and apoptosis rate were analyzed by flow cytometry. The expression of p21WAF1 gene was detected by immunohistochemistry. The inhibitory effect of DADS on K562 cells in a concentration range of 10 mg / L to 80 mg / L in a dose-time-dependent manner; 2) DADS treated K562 cells in different concentrations for 24 h, the cell cycle changed: DADS can block K562 cells in G2 / M phase; 3) After treated with DADS at concentration of 10mg / L ~ 80mg / L for 24 hours, the apoptosis rate of K562 cells gradually increased, with significant statistical significance (P <0.05 Or P <0.01); 4) The expression of p21WAF1 protein was up-regulated in K562 cells treated with 0mg / L, 20mg / L, 40mg / L and 80mg / 0.01). There was no difference between the vehicle group and the negative control group (P> 0.05). Conclusion: DADS can inhibit the proliferation of K562 cells and promote the apoptosis of K562 cells. Its possible mechanism is related to up-regulating cyclin-dependent kinase inhibitor p21WAF1 expression and inducing k562 cells to arrest in G2 / M phase.