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目的:探讨血管内皮生长因子(VEGF)蛋白及mRNA在乳腺癌发生、发展过程中的临床意义。方法:运用免疫组化EnvisionTM和半定量RT-PCR的方法检测56例乳腺癌和30例乳腺纤维腺瘤组织中VEGF蛋白及mRNA的表达情况,并分析其与临床病理参数的关系。结果:VEGF蛋白在乳腺癌组织中阳性表达率为75.0%(42/56),在乳腺纤维腺瘤组织中为23.3%(7/30);mRNA VEGF在乳腺癌和乳腺纤维腺瘤组织中表达水平分别为(0.8207±0.1042)、(0.3275±0.0384)灰度比值,差异有统计学意义(P<0.05);VEGF蛋白及mRNA的表达与组织学分级和淋巴结转移呈正相关(P<0.05)。结论:VEGF蛋白及mRNA参与乳腺癌的发生、发展过程,可作为评估乳腺癌浸润与淋巴结转移的重要生物学指标。
Objective: To investigate the clinical significance of vascular endothelial growth factor (VEGF) protein and mRNA in the occurrence and development of breast cancer. Methods: The expressions of VEGF protein and mRNA in 56 cases of breast cancer and 30 cases of breast fibroadenoma were detected by immunohistochemical EnvisionTM and semi-quantitative RT-PCR, and their relationship with clinicopathological parameters was analyzed. Results: The positive rate of VEGF protein in breast cancer tissues was 75.0% (42/56) and 23.3% (7/30) in breast fibroadenoma tissues. The mRNA expression of VEGF in breast cancer and breast fibroadenoma tissues (0.8207 ± 0.1042) and (0.3275 ± 0.0384), respectively. The difference was statistically significant (P <0.05). The expression of VEGF protein and mRNA was positively correlated with histological grade and lymph node metastasis (P <0.05). Conclusion: VEGF protein and mRNA are involved in the occurrence and development of breast cancer, which can be used as an important biological index to evaluate the infiltration and lymph node metastasis of breast cancer.