论文部分内容阅读
目的对比分析丙型肝炎患者血清自身抗体、HCV RNA水平和AST/ALT比值,以进一步探讨丙型病毒性肝炎的发病机理。方法采用间接免疫荧光法测定抗核抗体(ANA)、抗平滑肌抗体(SMA)、抗线粒体抗体(AMA)和抗肝肾微粒体抗体(LKM),采用核酸扩增(PCR)荧光定量技术检测标本HCV RNA含量,并同时检测肝功能指标丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)。结果HCV RNA阳性组和HCV RNA阴性组自身抗体检出率差异无统计学意义(P>0.05);自身抗体阳性组和自身抗体阴性组中AST/ALT比值≥1检出率有显著性差异(P<0.01);HCV RNA阳性组和HCVRNA阴性组AST/ALT比值≥1检出率有显著性差异(P<0.01)。结论病毒的直接作用和自身免疫是HCV损伤机体的两个重要因素。
Objective To compare and analyze serum autoantibodies, HCV RNA and AST / ALT ratio in patients with hepatitis C to further explore the pathogenesis of hepatitis C virus. Methods ANA, SMA, AMA and LKM were determined by indirect immunofluorescence method. The samples were detected by fluorescence quantitative PCR HCV RNA content, meanwhile detected ALT and AST. Results There was no significant difference in the detection rate of autoantibodies between HCV RNA positive group and HCV RNA negative group (P> 0.05). The detection rate of AST / ALT ratio ≥1 was significantly different between autoantibody positive group and autoantibody negative group P <0.01). There was a significant difference in the detection rate of AST / ALT ≥ 1 between HCV RNA positive group and HCVRNA negative group (P <0.01). Conclusion The direct effects of virus and autoimmunity are two important factors of HCV in the body.