论文部分内容阅读
该文采用荧光光谱法并用溴化乙锭（ＥＢ）作为荧光探针研究了道诺霉素（ＤＲＮ） 与ＤＮＡ相互作用的方式。结果表明：ＤＲＮ 的生色团能够嵌入ｄｓ ＤＮＡ 双螺旋结构的碱基对之间，ＤＲＮ 与ＤＮＡ 的主要作用位点是碱基Ｇ、Ｃ；ＤＲＮ 插入ｄｓ ＤＮＡ 碱其对中，不会破坏碱基对中氢键，不会影响ＤＮＡ 的复性；ＤＮＡ 与ｓｓ ＤＮＡ 仅仅存在弱的相互作用
In this paper, the interaction between daunorubicin (DRN) and DNA was studied by fluorescence spectroscopy and using ethidium bromide (EB) as a fluorescent probe. The results showed that the chromophore of DRN can intercalate between the base pairs of ds DNA double helix. The main sites of DRN and DNA are bases G and C. The insertion of dsDNA into DRN will not damage the base Base pairs of hydrogen bonds, DNA will not affect the renaturation; DNA and ss DNA only weak interaction