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目的 研究卡维地洛及肿瘤坏死因子α(TNF α)对内皮细胞分泌组织纤溶酶原激活物 (t PA)和纤溶酶原激活物抑制剂 1(PAI 1)的影响。方法 培养内皮细胞株 (ECV3 0 4) ,分为TNF α刺激组 ,培养基中TNF α加至终浓度为 5、10、2 5、5 0、10 0ng/ml;卡维地洛干预组 ,培养基中加TNF α (5 0ng/ml)后加入卡维地洛 ,终浓度为 2 0、5 0、10 0、2 0 0nmol/L ,2 4h后测定上清中的组织纤溶酶原激活物 (t PA)和纤溶酶原激活物抑制剂 1(PAI 1)的含量。结果 内皮细胞在TNF α刺激 2 4h后 ,其分泌的PAI 1抗原含量与对照组比有明显升高 ,有显著性差异 (P <0 0 5 ) ,而两组间t PA含量无明显差异 (P >0 0 5 )。而卡维地洛干预组却显著降低PAI 1(P <0 0 5 ) ,对t PA含量无明显作用 (P >0 0 5 )。结论 TNF α对内皮细胞株分泌的PAI 1有显著的升高作用 ,而对t PA无显著影响 ;用卡维地洛干预后 ,PAI 1显著降低 ,t PA含量无明显改变。
Objective To investigate the effects of carvedilol and tumor necrosis factor α (TNFα) on the secretion of tissue plasminogen activator (t PA) and plasminogen activator inhibitor 1 (PAI 1) by endothelial cells. Methods Endothelial cell line (ECV3 0 4) was cultured and divided into TNFα stimulation group, and TNFα was added to the final concentration of 5, 10, 25, 50, 100ng / ml in the carvedilol intervention group, After the medium was added with TNFα (50ng / ml), carvedilol was added to the medium at a final concentration of 20,5 0,10 0,2 0 0 nmol / L. Twenty four hours later, the level of tissue plasminogen Activators (t PA) and plasminogen activator inhibitor 1 (PAI 1) content. Results Compared with the control group, the level of PAI 1 antigen secreted by endothelial cells was significantly increased at 24 h after TNFα stimulation (P <0.05), while there was no significant difference between the two groups P> 0 0 5). Carvedilol intervention group, however, significantly reduced PAI 1 (P <0 05) and had no significant effect on t PA content (P 0 05). CONCLUSION: TNFα can significantly up-regulate the PAI 1 secretion in endothelial cell lines without any significant effect on t PA. After carvedilol intervention, PAI 1 is significantly decreased and t PA content is not significantly changed.