论文部分内容阅读
目的 研究肾血管性高血压大鼠蓝斑内NE能阳性神经元的变化。方法 雄性SD大鼠 6 0只 ,随机分为两组 (n =30 )。手术组用钳夹肾动脉法复制高血压模型 ,对照组手术过程同手术组 ,但不上银夹。于术后 7d ,30d ,90d处死动物 ,取脑 ,ABC免疫组化染色。结果 对照组大鼠蓝斑内NE能神经元密度在术后 7d、30d无变化 ,术后 90d显著增加 ;手术组NE能神经元密度先减少 ,再增加。手术组和对照组NE神经元密度相比 ,术后 30d两组间无差异 ;术后 7d、90d手术组高于对照组 (P <0 .0 5 ) ;结论 NE能阳性神经元在高血压的发生过程中起一定作用
Objective To investigate the changes of NE-positive neurons in the locus cerulensis of renovascular hypertensive rats. Methods Sixty male SD rats were randomly divided into two groups (n = 30). In the operation group, the model of hypertension was replicated by the method of clamping the renal artery. The operation of the control group was the same as that of the operation group but not the silver clip. The animals were killed at 7d, 30d and 90d after operation, and the brain and ABC immunohistochemical staining were performed. Results The NE neuron density in the locus coeruleus of rats in the control group did not change at 7d and 30d after operation and significantly increased at 90d after operation. The NE neuron density in the control group decreased first and then increased. Compared with control group, neuronal density of NE group was not significantly different between the two groups at the 30th day after surgery. The level of NE neurons in the operation group was significantly higher than that of the control group at 7d and 90d (P <0. 05) The occurrence of a certain role in the process