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采用正交实验优选H2O2氧化降解壳聚糖的最佳工艺条件,得到最佳降解方案:30%H2O2用量10 mL,反应温度60℃,反应时间6 h,乙酸浓度2%。在此基础上用壳聚糖及低聚糖溶液作为激发子诱导杨树抗病性,当低聚糖浓度为10 mg/L时,PAL、β-1,3-葡聚糖酶、几丁质酶及木质素活性达到最高值分别为对照的3.41、3.89、3.12、2.56倍。SOD、CAT及POD活性分别为对照的4.01、2.59、2.65倍。壳聚糖最佳诱导浓度为20 mg/L,此时PAL、β-1,3-葡聚糖酶、几丁质酶及木质素酶活性分别为对照的3.15、3.85、3.02、2.56倍。SOD、CAT及POD活性分别为对照的3.81、2.43、2.59倍。相同浓度的低聚糖与壳聚糖相比诱导效果更好。
The optimum conditions for the degradation of chitosan by H2O2 were optimized by orthogonal experiments. The best degradation scheme was as follows: 30 mL of 30% H2O2, reaction temperature 60 ℃, reaction time 6 h and acetic acid concentration 2%. On this basis, the disease resistance of poplar was induced by chitosan and oligosaccharide solution. When the concentration of oligosaccharide was 10 mg / L, PAL, β-1,3-glucanase, chitinase And lignin activity reached the highest values were 3.41,3.89,3.12,2.56 times the control. The activities of SOD, CAT and POD were 4.01, 2.59 and 2.65 times of the control, respectively. The optimum concentration of chitosan was 20 mg / L. The activities of PAL, β-1, 3-glucanase, chitinase and ligninase were 3.15, 3.85, 3.02 and 2.56 times respectively. The activities of SOD, CAT and POD were 3.81,2.43,2.59 times of the control respectively. The same concentration of oligosaccharides induced better than chitosan.