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目的探讨肿瘤抑素(Tumstatin)对喉鳞癌细胞(Hep-2)的治疗作用。方法用Hep-2细胞株进行传代培养,台盼蓝法观察Tumstatin和顺铂对不同培养时间细胞的生长抑制情况;MTT法检测不同浓度的Tumstatin对Hep-2细胞存活率的影响,光镜观察细胞形态的变化;电镜和3′-原位末端标记法(TUNEL)检测Hep-2细胞凋亡的发生和形态学改变。结果①随着药物对Hep-2细胞作用时间的延长,细胞生存率明显降低,存在时间依赖性;②MTT检测显示,Tumstatin对细胞的增殖的抑制效应具有剂量依赖性;③光镜观察发现,药物作用组细胞出现病变;④电镜和TUNEL染色证实,Tumstatin对Hep-2细胞的抑制作用是以促进细胞凋亡为主。结论Tumstatin可能通过促进细胞凋亡发挥抗肿瘤的作用。
Objective To investigate the therapeutic effect of Tumstatin on Hep-2 cells. Methods Hep-2 cells were subcultured. Trypan blue staining was used to observe the inhibitory effect of Tumstatin and cisplatin on the growth of Hep-2 cells at different concentrations. MTT assay was used to observe the effect of different concentration of Tumstatin on the survival rate of Hep-2 cells. The morphological changes of Hep-2 cells were detected by electron microscopy and TUNEL. Results ① The survival rate of Hep-2 cells was significantly decreased with the time-dependent manner; ② MTT assay showed that the inhibitory effect of Tumstatin on cell proliferation was dose-dependent; ③The results of light microscopy showed that the drug The cells in the treated group appeared lesions. (4) Electron microscopy and TUNEL staining confirmed that the inhibitory effect of Tumstatin on Hep-2 cells was mainly based on the promotion of cell apoptosis. Conclusion Tumstatin may play an anti-tumor role by promoting apoptosis.