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目的从子鼠海马中核转录因子NF-κB的变化,来探讨丰富环境对孕期慢性应激子鼠学习能力的干预机制。方法建立孕期慢性应激大鼠模型,并采用丰富环境干预子鼠,利用水迷宫和Y-迷宫测定子鼠学习记忆能力、RTPCR和Western Blotting测定海马中NF-κB表达情况。结果模型组母鼠皮质酮增高,提示模型组大鼠处于应激状态。丰富环境后,子鼠血浆皮质酮降低(P<0.05);水迷宫中逃避潜伏期和跨平台次数、Y-迷宫中所需的训练次数和正确反应率情况均转好(P均<0.05);模型子鼠组海马NF-κB表达增高(P均<0.05)。结论丰富环境可以提高孕期受到应激因素影响的学习能力,与血浆皮质酮降低及海马NF-κB升高有关。
OBJECTIVE: To investigate the mechanism of environmental enrichment on learning ability of chronically stressed offspring in pregnancy from the changes of NF-κB in the hippocampus of offspring. Methods Chronic stress rat model was established during pregnancy. The rats were also enrolled in this study. Enriched mice were used as control. Water maze and Y-maze were used to assess learning and memory abilities of rats. RTPCR and Western Blotting were used to detect the expression of NF-κB in hippocampus. Results In the model group, the corticosterone in the female rats increased, suggesting that the model rats were in stress state. After enriching the environment, the plasma corticosterone of the offspring decreased (P <0.05); the evading latency and cross-platform number in the water maze were all better than those in the Y-maze (P all <0.05) The expression of NF-κB in hippocampus of model mice increased (all P <0.05). CONCLUSION: Enriching environment can improve learning ability affected by stress factors during pregnancy, which is related to the decrease of plasma corticosterone and the increase of NF-κB in hippocampus.