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本实验研究了掌叶大黄和波叶大黄对肾佳偿性肥大的影响,以求进一步了解大黄延缓慢性肾衰的机理。材料和方法一、动物:雄性 SD 大鼠,体重100~150g,氯胺酮麻醉(100mg/kg),经腹切口取左肾,去肾包膜和肾上腺后,切除之。冷生理盐水冲尽肾内积血,-80℃冻存待用。二、药物:掌叶大黄和波叶大黄醇提物,波叶大黄水提物均由中国药科大学陈琼华教授
This experiment was conducted to study the effects of Rhubarb and Rhubarb on renal recombination hypertrophy in order to understand the mechanism of rhubarb-induced chronic renal failure. Materials and Methods Animals: Male SD rats weighing 100-150 g were anesthetized with ketamine (100 mg/kg). The left kidney was removed by abdominal incision, and the kidney capsule and adrenal gland were removed. Cold saline flushed kidney blood, -80 °C frozen for later use. Drugs: Rhubarb and Rheum palmatum extracts, Rheum palmatum water extracts by Professor Chen Qionghua of China Pharmaceutical University