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目的：探讨促炎症细胞因子（ＩＬ－ ６ 、ＩＬ－ ８ 、ＴＮＦ－ α） 以及Ｔ 细胞亚群在小儿过敏性紫癜发病中的作用。方法：采用双抗体夹心ＥＬＩＳＡ 法检测３０ 例急性期、２１ 例恢复期及２８ 例健康儿童血清及外周血单个核细胞（ＰＢＭＣ） 培养上清液ＩＬ－ ６ 、ＩＬ－ ８ 及ＴＮＦ－ α水平，以及外周血Ｔ 细胞及其亚群的变化。结果：急性期血清及ＰＢＭＣ 上清液ＩＬ－ ６ 、ＩＬ－８ 、ＴＮＦ－ α水平明显高于恢复期和对照组，且以ＩＬ－ ８ 、ＴＮＦ－ α增高为显著，恢复期上述３ 种细胞因子仍高于对照组；外周血ＣＤ３ 、ＣＤ４ 、ＣＤ８ 阳性细胞百分率及淋转率急性期明显低于对照组，至恢复期除ＣＤ８ 阳性细胞百分率恢复正常外，ＣＤ３ 、ＣＤ４ 阳性细胞及淋转率仍低于正常儿；重型病儿血清ＩＬ－ ８ 、ＴＮＦ－ α显著高于轻型病儿，ＰＢＭＣ 诱生的ＴＮＦ－ α水平亦高于较轻型病儿。结论：过敏性紫癜存在多种细胞因子失调，ＩＬ－ ８ 、ＴＮＦ－ α等促炎症细胞因子可能是介导过敏性紫癜血管炎症损伤过程中的重要细胞因子。Ｔ 细胞亚群失调及其功能低下是过敏性紫癜重要发病因素之一。
Objective: To investigate the role of pro-inflammatory cytokines (IL-6, IL-8, TNF-α) and T cell subsets in the pathogenesis of Henoch-Schonlein purpura in children. Methods: Serum and peripheral blood mononuclear cells (PBMCs) IL-6, IL-8 and TNF-α in 30 acute, 21 convalescent and 28 healthy children were detected by double antibody sandwich ELISA. As well as changes in peripheral T cells and their subsets. Results: The levels of IL-6, IL-8 and TNF-α in serum and PBMC supernatant in acute phase were significantly higher than those in recovery phase and control group, and the levels of IL-8 and TNF- The percentage of CD3, CD4, CD8-positive cells and the rate of lymph node metastasis were significantly lower in the peripheral blood than those in the control group, except for the percentage of CD8 positive cells returned to normal in the recovery phase Still less than the normal children; severe ill children serum IL-8, TNF-α was significantly higher than the light sick children, PBMC induced TNF-α levels are also higher than the lighter type of sick children. CONCLUSIONS: There are many cytokines imbalance in purpura. The proinflammatory cytokines such as IL-8 and TNF-α may be important cytokines in the process of inflammatory injury of allergic purpura. T cell subsets disorders and dysfunction is one of the important pathogens of Henoch-Schonlein purpura.